Journal: bioRxiv

Article Title: Insights into the foraging Gene’s Influence on Mating Investments of Male Drosophila

doi: 10.1101/2024.07.14.603413

Figure Lengend Snippet: (A) Flies expressing lacZ which is inserted in for 06860 were immunostained with anti-lacZ (Green), anti-elav (RFP), and nc82 (blue) antibodies. Scale bars represent 100 μm. The discrepancy in lacZ signal intensity on the surface of the male brain is likely due to the dissection procedure, which may have compromised the integrity of the surface glial cells expressing lacZ. (B) The threshold of lacZ (left panel), nc82 (right panel) in male and female were marked by threshold function of ImageJ. (C) Quantification of foraging gene expression in female and male brains. Fluorescence of lacZ was normalized by nc82. Bars represent the mean lacZ fluorescence level with error bars representing SEM. Asterisks represent significant differences, as revealed by the Student’s t test. (*p<0.05, **p<0.01, ***p< 0.001). (D) SCOPE scRNA-seq datasets reveal cell clusters colored by expression of foraging (red), doublesex / fruitless (blue/green) in neurons. (E) LMD assays for male knockdown of foraging driven by fru-GAL4. (F) LMD assays for male knockdown of foraging driven by dsx-GAL4. (G) Schematic diagram of foraging gene involvement in LMD.

Article Snippet: Primary antibodies: Chicken anti-GFP (1:500, Invitrogen), rabbit anti-LacZ antibody (1:1000, Rockland), rat anti-elav (1:100, DSHB), mouse anti-Bruchpilot (nc82) (1:50, DSHB).

Techniques: Expressing, Dissection, Fluorescence, Knockdown